Bioresources and Bioprocessing

Table 4 Probability of hydrogen bonds formation at the decamer interface of the wild-type CadA and its mutants

From: Enhanced thermal and alkaline stability of L-lysine decarboxylase CadA by combining directed evolution and computation-guided virtual screening

Hydrogen bonds	WT	K477R	E445Q	F102V	K477R/ E445Q	K477R/E445Q/F102V/T88S
GLN32-GLU21’	28.6	63.2	7.5	0.2	3.7	0.3
ASN49-LEU107’	30.6	1.7	1.2	58.7	3.5	41
LYS434-SER87’	6.5	46.8	29.5	94.7	34.9	0.9
ARG441-LEU89’	39	79.3	87.7	71	63.4	83.3
ARG543-GLU104’	48.6	73	64.4	32.2	58.3	48.9
ARG551-LEU93’	31.3	29.4	16.3	7.4	36.2	36
ARG551-LEU96’	62.3	76.6	94.3	27.6	91.2	52.1

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