Table 2 S. cerevisiae strains used and constructed in this study
Strain name | Parent strain | Genotype/description | Source |
|---|---|---|---|
Ycarot-02 | BY4741 | Δho::TTPS1-tHMG1-PGAL7-PGAL2-CrtYB-TPGK1-TCYC1-CrtI-PGAL1-PGAL10-CrtE03M-TADH1, Δgal1-7::TADH1-CrtYB-PGAL10-PGAL1-CrtI-TCYC1, Δgal80::LEU | |
Y03 | Ycarot-02 | Δlpp1::PGAL1-BLH-TCYC1 | This study |
Y03-11 | Y03 | Δdpp1::PGAL7-CrtE03M-TTPS1 | This study |
Y03-12 | Y03 | PERG9::PHXT1 | This study |
Y03-13 | Y03 | Δrox1 | This study |
Y03-14 | Y03 | Δmot3 | This study |
Y03-15 | Y03 | Δypl062w | This study |
Y03-111 | Y03-11 | PERG9::PHXT1 | This study |
Y03-112 | Y03-11 | Δrox1 | This study |
Y03-113 | Y03-11 | Δmot3 | This study |
Y03-21 | Y03 | Δdpp1::PGAL2-YMR315W-TPGK1 | This study |
Y03-211 | Y03 | Δdpp1::TTPS1-YMR315W-PGAL7-PGAL2-tPOS5-TPGK1 | This study |
Y03-22 | Y03 | Δdpp1::PGAL2-ZWF1-TPGK1 | This study |
Y03-221 | Y03 | Δdpp1::TTPS1-ZWF1-PGAL7-PGAL2-tPOS5-TPGK1 | This study |
Y03-23 | Y03 | Δdpp1::PGAL2-STB5-TPGK1 | This study |
Y03-24 | Y03 | Δdpp1::PGAL2-POS5-TPGK1 | This study |
Y03-25 | Y03 | Δdpp1::PGAL2-tPOS5-TPGK1 | This study |
Y03-251 | Y03 | Δmot3::PGAL10-tPOS5-TADH1 | This study |
Y03-252 | Y03 | Δmot3::TADH1-tPOS5-PGAL10-PGAL1-CrtE03M-TCYC1 | This study |
Y03-31 | Y03 | Δdpp1::PGAL7-AKR-TTPS1 | This study |
Y03-32 | Y03 | Δdpp1::PGAL7-AKR1B10-TTPS1 | This study |
Y03-33 | Y03 | Δdpp1::PGAL7-ybbO-TTPS1 | This study |
Y03-331 | Y03 | Δdpp1:: TTPS1-ybbO-PGAL2-PGAL7-ybbO-TTPS1 | This study |
Y03-34 | Y03 | Δdpp1::PGAL7-ENV9-TTPS1 | This study |
Y03-341 | Y03 | Δdpp1:: TTPS1-ENV9-PGAL2-PGAL7-ENV9-TTPS1 | This study |
Y03-342 | Y03 | Δdpp1:: TTPS1-ybbO-PGAL2-PGAL7-ENV9-TTPS1 | This study |
Y03-35 | Y03 | Δdpp1::PGAL7-SPS19-TTPS1 | This study |
Y03-36 | Y03 | Δdpp1::PGAL7-IFA38-TTPS1 | This study |
Y03-37 | Y03 | Δdpp1::PGAL7-YMR226C-TTPS1 | This study |
Y03-38 | Y03 | Δenv9 | This study |
Y03-41 | Y03 | Δmot3::TADH1-tPOS5-PGAL10-PGAL1-ENV9-TCYC1 | This study |
Y03-42 | Y03-41 | Δdpp1::PGAL7-CrtE03M-TTPS1 | This study |
Y03-43 | Y03-41 | Δdpp1::PGAL2-CrtE03M-TPGK1-PGAL7-ybbO-TTPS1 | This study |
Y03-43( +) | Y03-43 | met15::MET15, his3::HIS3, ura3::URA3 | This study |