Mutant | 60 °C | 65 °C | ||
---|---|---|---|---|
kd (1/h) | t1/2 (h) | kd (1/h) | t1/2 (h) | |
WT-PulAR | 0.14 | 4.8 ± 0.2 | 0.28 | 2.5 ± 0.1 |
PulAR-A365V | 0.12 | 5.9 ± 0.5 | 0.17 | 4.1 ± 0.2 |
PulAR-V401C | 0.12 | 6.0 ± 0.1 | 0.17 | 4.1 ± 0.1 |
PulAR-A365V-V401C | 0.07 | 9.9 ± 0.2 | 0.10 | 6.7 ± 0.4 |
PulAR-A365V-V401C-T504V | 0.05 | 13.2 ± 0.1 | 0.08 | 9.2 ± 0.2 |
PulAR-A365V-V401C-T504V-H499A | 0.04 | 17.5 ± 0.1 | 0.07 | 10.3 ± 0.3 |