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Table 2 Half-lives of WT-PulAR and its variants at 6065 °C and 65 °C

From: Tailoring pullulanase PulAR from Anoxybacillus sp. AR-29 for enhanced catalytic performance by a structure-guided consensus approach

Mutant

60 °C

65 °C

kd (1/h)

t1/2 (h)

kd (1/h)

t1/2 (h)

WT-PulAR

0.14

4.8 ± 0.2

0.28

2.5 ± 0.1

PulAR-A365V

0.12

5.9 ± 0.5

0.17

4.1 ± 0.2

PulAR-V401C

0.12

6.0 ± 0.1

0.17

4.1 ± 0.1

PulAR-A365V-V401C

0.07

9.9 ± 0.2

0.10

6.7 ± 0.4

PulAR-A365V-V401C-T504V

0.05

13.2 ± 0.1

0.08

9.2 ± 0.2

PulAR-A365V-V401C-T504V-H499A

0.04

17.5 ± 0.1

0.07

10.3 ± 0.3