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Bioresources and Bioprocessing

Table 4 Catalytic efficiencies of WT-PulAR and its mutants at 60 °C and pH 6.0

From: Tailoring pullulanase PulAR from Anoxybacillus sp. AR-29 for enhanced catalytic performance by a structure-guided consensus approach

Mutant

vmax (μmol min−1 mg−1)

KM (mg mL−1)

kcat (s−1)

kcat/KM (mL mg−1 s−1)

WT-PulAR

31.6 ± 1.2

1.64 ± 0.20

52.7

32.1

PulAR-A365V

44.0 ± 2.3

1.52 ± 0.12

73.3

48.2

PulAR- V401C

48.8 ± 1.6

1.52 ± 0.50

81.3

54.2

PulAR-A365V-V401C

85.0 ± 2.5

1.24 ± 0.32

141.7

114.3

PulAR-A365V-V401C-T504V

92.9 ± 2.0

1.09 ± 0.11

154.8

142.0

PulAR-A365V-V401C-T504V-H499A

112.0 ± 3.1

0.77 ± 0.15

186.7

242.5

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