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Bioresources and Bioprocessing

Table 5 Catalytic efficiencies of WT-PulAR and its mutants at 60 °C and pH 5.0

From: Tailoring pullulanase PulAR from Anoxybacillus sp. AR-29 for enhanced catalytic performance by a structure-guided consensus approach

Mutant

vmax (μmol min−1 mg−1)

KM (mg mL−1)

kcat (s−1)

kcat/KM (mL mg−1 s−1)

WT-PulAR

25.6 ± 1.1

4.67 ± 0.12

42.7

9.1

PulAR-A365V

35.3 ± 0.3

3.96 ± 0.13

58.8

14.8

PulAR-V401C

37.3 ± 0.5

3.77 ± 0.20

62.2

16.8

PulAR-A365V-V401C

65.3 ± 1.2

3.19 ± 0.50

108.8

34.1

PulAR-A365V-V401C-T504V

76.0 ± 2.3

2.63 ± 0.28

126.7

48.2

PulAR-A365V-V401C-T504V-H499A

84.7 ± 1.6

1.47 ± 0.05

141.2

96.1

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