Fig. 6

Chromatogram of native SPI, SPI hydrolysate, as well as proteins and peptides with known molecular weights. a Chromatogram of proteins and peptides with known molecular weights; b comparison of the output time of native SPI components and SPI hydrolysate. The vertical lines correspond to the peaks of the markers on (a). The SPI hydrolysate was obtained by enzymatic hydrolysis of 20% SPI isolate Shansong 90 using commercial enzyme Protosubtilin G3x with protease activity 11.9 Units/g of SPI at 50 \(^{\circ }\)C with no buffering