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Table 1 Adsorption parameters of ArCel5 constructions on Avicel pH-101 and filter paper

From: A novel accessory protein ArCel5 from cellulose-gelatinizing fungus Arthrobotrys sp. CX1

Substrate

Avicel pH-101

Filter paper

 

ArCel5

ArCel5-LD

ArCel5-NG

ArCel5-D

ArCel5

ArCel5-LD

ArCel5-NG

ArCel5-D

Bmax(μmol/g)

0.25 ± 0.03

0.18 ± 0.01

0.33 ± 0.10

0.12 ± 0.00

0.34 ± 0.03

0.15 ± 0.00

0.46 ± 0.022

0.21 ± 0.00

Kd (μM)

0.48 ± 0.11

2.08 ± 0.30

1.41 ± 0.19

2.53 ± 0.24

0.27 ± 0.05

1.79 ± 0.19

1.08 ± 0.11

2.04 ± 0.19

Absolute specificity (L/g)a

0.52

0.09

0.23

0.05

1.26

0.083

0.43

0.10

Relative specifityb

10.4

1.8

4.6

1

12.6

0.83

4.3

1

  1. Bmax maximum binding capacity, Kd dissociation constant
  2. aBmax/Kd
  3. bAbsolute specificities normalized on ArCel5-D