Table 1 Primers used in this study
From: De novo biosynthesis of τ-cadinol in engineered Escherichia coli
Primer name | Sequence (5′–3′) |
|---|---|
MvaS-XbaI | CTATCTAGAAAGAGGAGATATAATGACCATTGGTATTGATAAAATCAGCT |
MvaS-SpeI-BamHI | TACGGATCCACTAGTTTAGTTGCGATAGCTGCGCACG |
MvaE-XbaI | GTATCTAGAAAGAGGAGATATAATGAAGACCGTTGTGATTATTGACG |
MvaE-SpeI-BamHI | TATGGATCCACTAGTTTACTGTTTACGCAGGTCGTTCAGG |
IspA-XbaI | GTATCTAGAAAGAGGAGATATAATGGACTTTCCGCAGCAACTCG |
IspA-SpeI-BamHI | TATGGATCCACTAGTTTATTTATTACGCTGGATGATGTAGTCCG |
CS-XbaI | GTATCTAGAAAGAGGAGATATAATGGCGACGAGCGCGGT |
CS-SpeI-BamHI | TATGGATCCACTAGTTTAAAACACCAGCGGATCTAAAAACA |
IdI-XbaI | ATCTCTAGAAAGAAGGAGATATAATGCAAACGGAACACGTCATTTTAT |
IdI-XhoI | ACATCTCGAGTTATTTAAGCTGGGTAAATGCAGATAATC |