Table 2 Plasmids and strains used in this study
From: De novo biosynthesis of τ-cadinol in engineered Escherichia coli
Plasmids | Properties | Copy number | Source |
|---|---|---|---|
pDG30 | pSC101 origin, AmpR, pT7: AtoB, ERG13 and tHMG1 | 1 | Guo et al. 2018 |
pDG31 | pBBRMCS1 origin, ChlR, pT7: IdI, ERG8, MVD1 and ERG12 | 10–20 | Guo et al. 2018 |
pSY09 | pSC101 origin, AmpR, pT7: MvaSA110G and MvaE | 1 | This study |
pSY12 | pBR322 origin, KanR, pT7: IspA and CS | 100–200 | This study |
pSY13 | pBR322 origin, KanR, pT7: IspA, CS and IdI | 100–200 | This study |
Strains | |||
SY01 | BL21(DE3) derivative: pSY09 carrying MvaSA110G and MvaE, pDG31 carrying IdI, ERG8, MVD1 and ERG12, and pSY12 carrying IspA and CS | This study | |
SY02 | BL21(DE3) derivative: pDG30 carrying AtoB, ERG13 and tHMG1, pDG31 carrying IdI, ERG8, MVD1 and ERG12, and pSY12 carrying IspA and CS | This study | |
SY03 | BL21(DE3) derivative: pDG30 carrying AtoB, ERG13 and tHMG1, pDG31 carrying IdI, ERG8, MVD1 and ERG12, and pSY13 carrying IspA, CS and IdI | This study | |