Fig. 5

Comparison of reducing power and degradation of feathers. a Reducing power (OD₇₀₀). 1, pMA5; 2, WT; 3, variant T18. b ATBS radical scavenging. 1, pMA5; 2, WT; 3, variant T18. c Degradation of feathers by combined enzymes. 1, WT; 2, variant T18; 3, papain and variant T18; 4, pepsin and variant T18; 5, trypsin and variant T18; 6, bromelain and variant T18; 7, flavor protease and variant T18; 8, neutral protease and variant T18; 9, alkaline protease and variant T18