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Table 2 The changes of amino acid concentration during feather degradation process by the variant T18

From: Directed evolution driving the generation of an efficient keratinase variant to facilitate the feather degradation

Amino acid

Concentration (mg/L)

0 h

12 h

24 h

36 h

asp

25.09 ± 0.31

44.05 ± 1.47

28.99 ± 1.80

23.71 ± 4.58

glu

41.12 ± 0.16

122.94 ± 3.16

11.85 ± 2.63

17.32 ± 4.12

ser

0.88 ± 0.80

12.99 ± 0.66

1.92 ± 1.32

0.80 ± 0.59

his

21.03 ± 1.82

269.73 ± 0.45

23.01 ± 1.70

17.31 ± 4.78

gly

22.16 ± 0.13

219.54 ± 6.42

56.85 ± 7.11

3.18 ± 0.27

thr

5.27 ± 0.21

917.09 ± 24.37

272.72 ± 5.64

537.47 ± 12.29

arg

11.31 ± 0.56

25.09 ± 0.43

0.58 ± 0.042

1.06 ± 0.51

ala

32.5 ± 0.62

23.80 ± 0.14

2.61 ± 0.70

3.64 ± 0.42

tyr

146.01 ± 2.09

387.42 ± 5.94

121.41 ± 4.24

114.32 ± 7.87

cys

6.38 ± 0.69

14.56 ± 1.47

4.84 ± 0.67

4.26 ± 0.50

val

165.18 ± 0.83

389.84 ± 22.32

22.86 ± 12.06

22.66 ± 2.60

met

116.92 ± 2.81

370.51 ± 15.36

72.61 ± 6.84

44.6 ± 6.52

phe

199.02 ± 0.95

688.07 ± 14.47

152.44 ± 14.56

154.41 ± 1.88

ile

63.84 ± 0.88

55.39 ± 1.64

1.80 ± 0.30

1.23 ± 0.24

leu

180.32 ± 1.63

349.90 ± 8.14

7.16 ± 1.28

6.57 ± 0.93

lys

282.93 ± 5.09

1066.35 ± 13.87

187.07 ± 6.01

149.06 ± 13.82

pro

231.74 ± 12.78

14.43 ± 1.70

113.69 ± 16.87

111.81 ± 12.49