Fig. 5From: High coenzyme affinity chimeric amine dehydrogenase based on domain engineeringEffect of NAD+ concentration on the reductive amination of pFPA. a 0.05 mM NAD+; b 0.5 mM NAD+. Reaction conditions: pFPA (0.2 M), NH4Cl–NH3H2O (2 M), glucose (0.24 M) and purified cFLF-AmDH and F-BbAmDH. (1 gL−1) in Tris–HCl buffer (0.1 M, pH 8.5), 30 °C, 200 rpmBack to article page