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Table 1 Kinetic parameters of the parent for substrate NADH

From: High coenzyme affinity chimeric amine dehydrogenase based on domain engineering

Enzyme

Substrate

Km (mM)

kcat (min−1)

kcat/Km (mM−1 min−1)

F-BbAmDH

NADH

0.16 ± 0.02

0.65 ± 0.01

4.03

L-BcAmDH

NADH

0.021 ± 0.004

1.09 ± 0.1

51.40

L-EsAmDH

NADH

0.052 ± 0.005

1.62 ± 0.22

31.15

  1. Determination of coenzyme kinetic parameters using the same substrate 2-pentanone. The values were generated by fitting the initial specific activity data to the Michaelis–Menten equation using nonlinear regression with GraphPad Prism software. Value is means ± standard deviations. All reactions involved in the kinetic constant calculations were analyzed using a 2 M NH4Cl/NH4OH buffer at optimum pH and temperature. All experiments were repeated 3 times