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Table 4 Specific activity (mU mg−1) of F-BbAmDH and cFLF-AmDH toward aromatic ketones

From: High coenzyme affinity chimeric amine dehydrogenase based on domain engineering

Substrates

Specific activity (mU mg−1)a

F-BbAmDH

cFLF-AmDH

Acetophenone

n.a.b

21.96

2-Methylacetophenone

n.a

n.a

3-Methylacetophenone

n.a

23.32

4-Methylacetophenone

n.a

n.a

4-Fluoroacetophenone

n.a

n.a

3-Hydroxyacetophenone

n.a

n.a

4-Methoxyacetophenone

n.a

n.a

4-Fluorophenylacetone

719.8

1759.4

4-Methylpropiophenone

377.9

838.1

4-Hydroxypropiophenone

n.a

n.a

P-Methoxyphenylacetone

199.1

337.9

4-(4-Methoxyphenyl)-2-butanone

n.a

n.a

  1. aActivity was measured in NH4Cl/NH4OH buffer (2 M, pH 9.0) containing 0.2 mM NADH and 20 mM substrate at 30 °C
  2. bn.a. = no measurable activity