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Bioresources and Bioprocessing
Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: Salt-tolerant and thermostable mechanisms of an endoglucanase from marine Aspergillus niger

Fig. 1

Constitutive expression and purification. A Constitutive expression in GPY medium. Lane M: protein marker; lane 1: concentrated supernatant of original strain; lane 2: concentrated supernatant of recombinant. B Purification of AnEGL by anion exchange chromatography. Lane M: protein marker; lane 3: ultrafiltrate; lane 4: wash with 20 mM pH 7.0 sodium phosphate buffer; lanes 5 to 8: elution with 0.1, 0.2, 0.3, 0.5 M NaCl

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