Fig. 5

The function of NaCl on salt tolerance and thermostability. A The spatial position of Na⁺ before MD simulation at 300 K in the absence of NaCl. B The spatial position of Na⁺ after 5 ns MD simulation at 300 K in the absence of NaCl. The red sphere represents Na⁺. C Surface electrostatic potential of AnEGL. The region circled with the ellipse is the substrate binding pocket. Electrostatic potential between − 5 kT/e and 5 kT/e is shown as colored gradient from red to white to blue. D Charged residues of AnEGL. The electropositive and electronegative residues are colored with blue and red, respectively