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Bioresources and Bioprocessing

Table 1 Biotransformation of rutin to quercetin

From: Bioproduction of quercetin using recombinant thermostable glycosidases from Dictyoglomus thermophilum

Recombinant biocatalyst

Substrate concentration

(g/L)

Quercetin yield

(g/L)

Isoquercetin yield

(g/L)

Conversion rate

(mol product/mol substrate)

Strain harboring plasmid of pRSFDuet-DthRha

10

N.A

7.30 ± 0.14

0.96 ± 0.18

Strain harboring plasmid of pRSFDuet-Dth3

10

N.A

N.A

N.A

Strain harboring plasmid of pRSFDuet-Dth3-DthRha

10

5.87 ± 0.31

N.A

1.19 ± 0.06

  1. The substrate rutin contained a trace quantity of quercetin, which was not subtracted in the calculations of product yield and conversion rate
  2. N.A. indicates that the data are unavailable due to a low concentration of the target compound that exceeds the HPLC detection limit or the absence of the target chemical in the sample
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