Fig. 5
From: Catalytic properties and biological function of a PIWI-RE nuclease from Pseudomonas stutzeri
Effects of ciprofloxacin on the morphology of wild-type and △piwi-re strains of P. stutzeri. A The growth of wild-type P. stutzeri and △piwi-re strains in the presence of ciprofloxacin. The wild-type and △piwi-re strain cultures were grown to an optical density (OD600) of 0.5 before being divided into two groups. One group was treated with 5 μM ciprofloxacin for 6 h, while the other group was not treated with ciprofloxacin and was the control. Experiments were performed in triplicate and representative transmission electron microscope images are shown. B Quantitative analysis of the effect of ciprofloxacin on strain length of wild-type and △piwi-re strains of P. stutzeri. The lengths of 50 cells in each group were counted using Digital Micrograph software (Gatan, USA), The data were analyzed in GraphPad Prism 8. Mean values ± SD are shown (n = 50, **p = 0.0010). The p value was calculated using an unpaired Student’s t test