Fig. 2
From: A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis
Key DNA sequence before and after the gene of interest (A), plasmid map of pHT7-GFP (B) and scheme of plasmid multimerization (C). Plasmid pHT7-GFP contains the T7 promoter-terminator cassette including a lacO lactose operator and a SD sequence, the replication origin ori in E. coli, the replication origin repA in B. subtilis, the lac repressor gene, chloramphenicol-resistance gene and ampicillin-resistance gene