From: A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis
Promoter | Inducer | Characteristics | References |
---|---|---|---|
PxylA | Xylose | Strictly controlled by XylR repressor, and the protein expression was inhibited by glucose | |
PmalA | Maltose | Positively regulated by the transcriptional regulator MalR, and was repressed by glucose via CcpA and catabolite responsive elements | |
PspaS | Subtilin | The level of expression depended directly on the amount of inducer (subtilin) used, not subject to catabolite control | (Bongers et al. 2005) |
Pgcv | Glycine | The glycine tandem riboswitch was used to obtain regulatable expression of recombinant proteins | (Phan and Schumann 2007) |
Pspac | IPTG | Fused the 5ʹ-sequence of a promoter from the B. subtilis phage SPO1 and the 3ʹ-sequences of the E. coli lac promoter including its operator region | (Yansura and Henner 1984) |
Pgrac | IPTG | Fused groES promoter and lacO operon and optimization of nucleotides at the conserved regions of the groESL promoter |