From formic acid to single-cell protein: genome-scale revealing the metabolic network of Paracoccus communis MA5

Bioresources and Bioprocessing

Table 1 Physiological parameter detection of strain MA5 under different culture conditions

Sample	Formic acid absorption (g/L)	Biomass (OD₆₀₀)	Protein content (%)
A	4.61 ± 0.24	0.56 ± 0.07	44 ± 1.55
B	1.51 ± 0.22 ^a	0.49 ± 0.09^b	30 ± 1.79^a
C	4.40 ± 0.37 ^b	0.21 ± 0.05^a	53 ± 1.31^a

Culture condition A: 8 g/L HCOONa, 1 g/L yeast powder, 0.5 g/L NaCl, 0.5 g/L MgCl₂.6H₂O, 0.1 g/L CaCl₂.2H₂O, 0.5 g/L KCl, 0.2 g/L KH₂PO₄. Culture condition B: 20 g/L HCOONa, 1 g/L yeast powder, 0.5 g/L NaCl, 0.5 g/L MgCl₂.6H₂O, 0.1 g/L CaCl₂.2H₂O, 0.5 g/L KCl, 0.2 g/L KH₂PO₄. Culture condition C: 8 g/L HCOONa, 2 g/L (NH4)₂SO₄, 0.5 g/L NaCl, 0.5 g/L MgCl₂.6H₂O, 0.1 g/L CaCl₂.2H₂O, 0.5 g/L KCl, 0.2 g/L KH₂PO₄. Error bars ( ±) represent the standard deviation of three replicates. Letters indicate statistically significant differences, as determined by Student’s t test (^aindicates p < 0.01; ^bindicates no significance)