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Bioresources and Bioprocessing
Fig. 13 | Bioresources and Bioprocessing

Fig. 13

From: Atomevo: a web server combining protein modelling, docking, molecular dynamic simulation and MMPBSA analysis of Candida antarctica lipase B (CalB) fusion protein

Fig. 13

SDS-PAGE result (left) and western blot (right) of MT-CalB. Lane M1: protein marker; Lane M2: western blot Protein marker; Lane PC1: BSA (1 μg); Lane PC2: BSA (2 μg); Lane NC: uninduced whole cell; Lane 1: whole cells were induced at 26 oC for 16 h; Lane 2: whole cells were induced at 15 oC for 16 h; Lane NC1: uninduced pericytoplasm; Lane 3: periplasmic cells were induced at 26 oC for 16 h; Lane 4: pericytoplasm was induced at 15 oC for 16 h; Lane NC2: uninduced supernatant of cell lysis; Lane 5: induced cell lysis supernatant at 26 oC for 16 h; Lane 6: cell lysis supernatant was induced at 15 °C for 16 h; Lane NC3: uninduced cell lysis precipitation; Lane 7: 26 °C Induced cell lysis precipitation for 16 h; Lane 8: induced cell lysis precipitation for 16 h at 15 °C

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