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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: A modular 3D printed microfluidic system: a potential solution for continuous cell harvesting in large-scale bioprocessing

Fig. 3

Harvesting MSCs with our microfluidic system. The concentrating efficiency of the zig-zag channel is shown in Additional file 1: Fig. S9. A and B Fluorescent microscopy images of cells–MCs before and after passing through the mixer. Cell nuclides were stained with Hoechst. C Separation of cells and microcarriers through the spiral chip. Cells and MCs were separated through different channels based on their size difference. D The recovery rate of cells and MCs after passing through the spiral chip in one round. The liquid collected from the inner outlet of the spiral was collected and performed a second-round running through to further recover the cells. The two-round separation results are shown in Additional file 1: Fig. S8

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