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Bioresources and Bioprocessing
Fig. 5 | Bioresources and Bioprocessing

Fig. 5

From: A modular 3D printed microfluidic system: a potential solution for continuous cell harvesting in large-scale bioprocessing

Fig. 5

MSCs characterisation after harvesting. A Expression of the MSCs surface markers CD90, CD73, and CD105 after 3 passages of indicated cells preserve their stemness after harvesting. B Multipotency assay of harvested cells using Oil red (left), Alizarin red (middle), and Alcian blue (right) showed the cells maintained their capacity to differentiate into different cell types. C The expression level of the surface therapeutic proteins of the experimental group. The changes in the expression level of HLA-G and CD54 were similar in both groups. D Comparison of the cytokine secretion profile of MSCs harvested from the microcarriers with microfluidic system and the passage 4, passage 8 planar flask cultured cells

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