Fig. 1
From: Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris
Construction of α-alkenes biosynthetic pathway in P. pastoris. a Schematic illustration of metabolic pathways engineered for the production of α-alkenes from FFA via three pathways. b FFA titers of faa1Δ strain in shake flask after 96 h cultivation in 20 g/L glucose medium or 120 h cultivation in 20 g/L methanol medium. c α-Alkenes production from three different decarboxylases in glucose medium. d α-Alkenes profiles of the engineered strain with expression of different decarboxylases in glucose medium. C15:1, 1-pentadecene; C17:1, 1-heptadecene; C17:2, 1,8-heptadecadiene. e Comparison of episomal expression and chromosome integration of UndB for α-alkenes production. All data were represented as the mean ± s.d. of three samples