Skip to main content
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris

Fig. 3

Fermentation of α-alkenes from methanol as sole carbon resource. a Total α-alkenes production in corresponding strains in shake flask after 120 h cultivation in 20 g/L methanol medium. b The relative expression levels of UndB under different promoters. The housekeeping gene actin was chosen as baseline for quantification of expression level. Relative expression levels of UndB were calculated by normalized the expression level under PGAP. All data were represented as the mean ± s.d. of three samples. Statistical significance of the different α-alkenes levels in comparison with the control (PGAP) was evaluated using Student’s t test (*P < 0.05; **P < 0.01)

Back to article page