Fig. 4From: A multi-component reaction for covalent immobilization of lipases on amine-functionalized magnetic nanoparticles: production of biodiesel from waste cooking oilEffect of temperature on the activity of free and immobilized lipases. The incubation at different temperatures was carried out in 25Â mM sodium phosphate buffer, pH 7.0. The initial activity of each derivative before incubation was determined and set as 100%Back to article page