Table 1 Immobilization of the lipases on amine-functionalized magnetic nanoparticles
Offered enzyme (mg/g) | Yield (%) | Immobilized enzyme (mg/g support) | Immobilization time (h) | Specific activity U/mg (recovered activity) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Â | RML | CALB | TLL | RML | CALB | TLL | RML | CALB | TLL | RML | CALB | TLL |
20 | 100 | 99.2 | 98.5 | 20.0 | 19.8 | 19.7 | 2 | 2 | 2 | 8.2 (142) | 35.0 (157) | 26.8 (101) |
40 | 100 | 98.5 | 98.7 | 40.0 | 39.4 | 39.5 | 2 | 3 | 3 | 7.5 (125) | 30.1 (112) | 23.4 (95) |
60 | 98.5 | 95.0 | 98.3 | 59.1 | 57.0 | 58.9 | 3 | 5 | 5 | 6.6 (90) | 24.6 (72) | 18.3 (99) |
80 | 96.0 | 92.32 | 93.7 | 76.8 | 73.8 | 75.0 | 12 | 12 | 12 | 5.3 (93) | 18.9 (74) | 13.7 (100) |
100 | 97.5 | 94.0 | 99.5 | 97.5 | 94.0 | 99.5 | 12 | 12 | 12 | 4.2 (77) | 9.3 (56) | 9.8 (70) |