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Bioresources and Bioprocessing

Table 1 The absorption, desorption, and recovery of 5-ALA from cation ion-exchange chromatography using different eluents

From: Purification and biofabrication of 5-aminolevulinic acid for photodynamic therapy against pathogens and cancer cells

Eluents

Conc. (M)

pH

Applied volume (mL)

Adsorbeda 5-ALA (g)

Desorbed 5-ALA (g)

Recovery (%)

HCl

1.5

ND

110

1.24

0.101

8.1

HCl

3.0

ND

110

1.24

0.149

12.0

CH3COONa

0.74

3.1

110

1.24

0.78

62.9

CH3COONa

0.74

3.1

250

1.24

0.79

63.7

NH4OH

1.0

11.5

250

1.25

1.15

92.0

  1. aAll the chromatography is injected by 300 mL 5-ALA at 4.16 g/L for each batch. ND means not determined
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