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Bioresources and Bioprocessing

Table 2 The purity of 5-ALA from different solvents extraction and precipitation

From: Purification and biofabrication of 5-aminolevulinic acid for photodynamic therapy against pathogens and cancer cells

Organic solvents

Solvent volume (mL)

5-ALA volume (mL)

Crude product (g)

5-ALA content (g)

Purity (%)

Diethyl ether

20

2

0.001

ND

ND

Methanol

20

2

0.12

0.005

4.17

Ethanol

20

2

0.30

0.007

2.33

Acetone

10

2

0.36

0.13

36.1

Acetone

20

2

0.50

0.25

50.0

Acetone

40

2

0.45

0.21

46.7

  1. a5-ALA is used at concentration of 500 g/L. ND means not determined
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