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Bioresources and Bioprocessing
Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: Excellent removal of knob-into-hole bispecific antibody byproducts and impurities in a single-capture chromatography

Fig. 2

AKTA chromatograms and HPLC-SEC chromatograms of elute fractions of MabSelect PrismA runs. AKTA chromatogram of FabscFv-KiH (a) and Fab2scFv-KiH (b), with a pH gradient elution from pH 6.0 to pH 3.0 in 25 CV using MabSelect PrismA resin. The pH values indicated here represent the pH of each fraction measured by an external pH probe, with each fraction number indicated at the top of the chromatogram. HPLC-SEC chromatogram of FabscFv-KiH (c) and Fab2scFv-KiH (d) of each fraction of the eluates with absorbance values normalized to that of the monomeric peak, where each absorbance value was divided by the highest absorbance value of the monomeric peak. The likely hole–hole homodimeric mispaired products are indicated with*. The purity profile of the pooled post-Protein A eluate of all 9 fractions with respect to the CCS is also illustrated for FabscFv-KiH (e) and Fab2scFv-KiH (f)

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