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Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Excellent removal of knob-into-hole bispecific antibody byproducts and impurities in a single-capture chromatography

Fig. 3

AKTA chromatograms of MabSelect PrismA runs with a 2-step pH elution. A 2-step elution of pH 3.8 and pH 3.6 for FabscFv-KiH (a) and Fab2scFv-KiH (b), respectively, and a 2-step elution of pH 3.6 and pH 3.4 for FabscFv-KiH (c) and Fab2scFv-KiH (d), respectively, with the higher and lower pH eluate analyzed highlighted in light and dark grey, respectively

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