Elution conditions | Monomer concentration (mg/mL) | Monomer recovery (%) | Purity (%) | ||||
---|---|---|---|---|---|---|---|
HMW | Mono | LMW | |||||
FabscFv-KiH | CCS | – | 0.70 | – | 34.9 | 28.9 | 36.2 |
MabSelect PrismA eluate | pH gradient elution from pH 6.0 to pH 3.0 in 25 CVs | 1.02 | 101.1 | 8.5 | 90.9 | 0.6 | |
2-step elution: | |||||||
pH 3.8 mock pool | 0.21 | 44.4 | 1.7 | 97.6 | 0.8 | ||
pH 3.6 mock pool | 0.50 | 50.1 | 6.0 | 94.0 | 0.1 | ||
2-step elution: | |||||||
pH 3.6 mock pool | 0.78 | 94.6 | 6.7 | 92.3 | 0.9 | ||
pH 3.4 mock pool | 0.02 | 0.8 | 61.8 | 26.7 | 11.5 | ||
Fab2scFv-KiH | CCS | – | 0.78 | – | 34.3 | 30.4 | 35.3 |
MabSelect PrismA eluate | pH gradient elution from pH 6.0 to pH 3.0 in 25 CVs | 0.98 | 92.9 | 10.9 | 87.2 | 1.8 | |
2-step elution: | |||||||
pH 3.8 mock pool pH 3.6 mock pool | 0.24 | 51.2 | 5.1 | 89.8 | 5.1 | ||
0.52 | 43.9 | 6.8 | 91.1 | 2.1 | |||
2-step elution: | |||||||
pH 3.6 mock pool | 1.11 | 93.5 | 7.7 | 89.4 | 2.9 | ||
pH 3.4 mock pool | 0.02 | 0.6 | 47.4 | 24.4 | 28.2 |