Table 2 Effect of different loading amounts and residence time on the purity profile and recovery of post-Protein A eluates obtained with pH 3.6 elution
Loading conditions | Monomer concentration (mg/mL) | Monomer recovery (%) | Purity (%) | ||||||
|---|---|---|---|---|---|---|---|---|---|
QB10 (%) | Residence time (min) | Total loading time (h) | HMW | Mono | LMW | ||||
FabscFv-KiH | CCS | – | – | – | 0.52 | – | 29.5 | 28.3 | 42.2 |
MabSelect PrismA eluate | 80 | 6 | 9.7 | 2.77 | 82.4 | 14.2 | 85.3 | 0.5 | |
50 | 6 | 6.0 | 2.59 | 90.4 | 11.0 | 88.5 | 0.5 | ||
50 | 2 | 6.0 | 2.59 | 90.4 | 11.2 | 88.3 | 0.5 | ||
Fab2scFv-KiH | CCS | – | – | – | 0.72 | – | 31.1 | 33.5 | 35.4 |
MabSelect PrismA eluate | 50 | 6 | 4.2 | 2.14 | 91.2 | 8.4 | 89.1 | 2.5 | |