Table 4 Validation runs for both FabscFv-KiH and Fab2scFv-KiH in 5-mL columns
Conditions | Monomer concentration (mg/mL) | Monomer recovery (%) | Purity (%) | |||||
|---|---|---|---|---|---|---|---|---|
HMW | Mono | LMW | ||||||
5-mL column | FabscFv-KiH | CCS | – | 0.69 | – | 30.8 | 35.5 | 33.8 |
MabSelect PrismA | FT | 0.02 | 2.8 | |||||
pH 6.0 wash | 0.01 | 0.1 | ||||||
pH 4.7 wash | 0.02 | 0.6 | 5.4 | 51.5 | 43.1 | |||
pH 3.6 peak (UV280 > 50 mAu) | 5.00 | 91.1 | 5.7 | 93.9 | 0.4 | |||
pH 3.6 tail (UV280 < 50 mAu) | 0.03 | 1.3 | 26.0 | 71.7 | 2.3 | |||
Fab2scFv-KiH | CCS | – | 0.72 | – | 33.5 | 31.1 | 35.4 | |
MabSelect PrismA | FT | 0.01 | 2.0 | – | – | – | ||
pH 6.0 wash | 0.01 | 0.1 | – | – | – | |||
pH 4.3 wash | 0.01 | 0.4 | 7.6 | 3.4 | 89.0 | |||
pH 3.6 peak (UV280 > 50 mAu) | 2.74 | 89.8 | 6.1 | 92.1 | 1.8 | |||
pH 3.6 tail (UV280 < 50 mAu) | 0.06 | 2.0 | 14.2 | 84.3 | 1.5 | |||
MabSelect PrismA | FT | 0.01 | 2.0 | – | – | – | ||
pH 6.0 wash | 0.01 | 0.1 | – | – | – | |||
pH 4.1 wash | 0.34 | 11.9 | 14.9 | 72.9 | 12.2 | |||
pH 3.6 peak (UV280 > 50 mAu) | 2.92 | 78.4 | 6.5 | 92.1 | 1.4 | |||
pH 3.6 tail (UV280 < 50 mAu) | 0.03 | 1.2 | 51.3 | 46.0 | 2.7 | |||