Table 6 Genes involved in Cd, Cr, As and Pb resistance in B. amyloliquefaciens strain Bam1 and B. velezensis strain FZB42
Metal | Gene | Location | Product or function | Identity of FZB42 (%) | |
|---|---|---|---|---|---|
Bam1 | FZB24 | ||||
Cd | czrA | 1,940,021–1,940,323 | 2,011,551–2,011,933 | ArsR family transcriptional repressor, regulation of resistance against toxic metal cations; regulating the expression of cadA, czcD | 97.69 |
|  | cadA | 3,237,502–3,239,613 | 3,183,406–3,185,517 | Cd-translocating P-type ATPase; Cd2+/Zn2+/Co2+ efflux | 93.51 |
|  | czcD | 506,576–507,511 | 537,946–538,876 | Cation exporter (antiporter); cation efflux; resistance against Zn2+, Cu2+, Co2+, Ni2+, Cd2+ | 89.40 |
|  | pfeT/zntA/zosA | 1,370,249–1,372,162 | 1,321,623–1,323,536 | Fe2+/Zn2+/Cd2+/Pb2+-exporting ATPase; cation efflux | 91.54 |
|  | arsR | 1,940,021–1,940,323 | 1,978,959–1,979,261 | ArsR family transcriptional regulator; regulation the expression of arsB, may have the potential to sequester Cd | 97.69 |
|  | arsB | 3,494,481–3,495,812 | 3,687,147–3,688,445 | Arsenical pump membrane protein; arsenite exporter, may efflux Cd2+ | 93.32 |
Cr | chrS/ywrC | 3,502,399–3,502,875 | 3,450,892–3,451,368 | AsnC family transcriptional repressor; regulation of chromate export | 94.55 |
|  | chrA1/ywrB | 3,502,889–3,503,479 | 3,451,382–3,451,972 | Chromate transporter ChrA1/YwrB; chromate exporter; chromate detoxification | 89.00 |
|  | chrA2/ywrA | 3,503,497–3,504,012 | 3,451,990–3,452,505 | Chromate transporter ChrA2/YwrA; chromate exporter; chromate detoxification | 92.64 |
As | arsR | 1,940,021–1,940,323 | 1,978,959–1,979,261 | ArsR family transcriptional regulator; regulation the expression of arsB and arsC | 97.69 |
|  | arsB | 3,494,481–3,495,812 | 3,687,147–3,688,445 | As3+ pump membrane protein; As3+ export | 93.32 |
|  | arsC | 3,160,891–3,161,247 | 3,105,252–3,105,608 | As5+ reductase | 96.64 |
|  | aseR | 3,729,932–3,730,282 | 3,686,783–3,687,133 | ArsR family transcriptional repressor; regulation of As3+ efflux pump ArsA | 89.74 |
|  | arsA/ydfA/yqcL | 3,730,296–3,731,594 | 3,687,147–3,688,439 | As3+ pump membrane protein; As3+ efflux pump; detoxification of As3+ and As5+ | 93.35 |
Pb | cadA | 3,237,502–3,239,613 | 3,183,406–3,185,517 | Cd-translocating P-type ATPase; Cd2+/Zn2+/Co2+ efflux; Pb2+ efflux in S. aureus | 93.51 |
|  | pfeT/zntA/zosA | 1,370,249–1,372,162 | 1,321,623–1,323,536 | Fe2+/Zn2+/Cd2+-exporting ATPase; cation efflux; Pb2+ efflux in E. coli | 91.54 |