Fig. 4
From: Production of free fatty acids from various carbon sources by Ogataea polymorpha
Co-feed of glucose and methanol for FFA production in strain FD09. A Schematic diagram of glucose and methanol metabolism in O. polymorpha. Supplemented glucose will provide extra NADPH for both cell growth and FFA production. B Concentration of carbon sources (-M: methanol concentration, -G: glucose concentration, 10 M-c: control of methanol volatilization without inoculation) in different carbon sources. 20G: 20 g/L glucose, 10 M: 10 g/L methanol, 20G + 10 M: 20 g/L glucose and 10 g/L methanol, 20G + 10 M-a: 20 g/L glucose with 10 g/L methanol supplemented at 24 h. C Growth curve of FD09 in different carbon sources. D FFA titer of FD09 in different carbon sources. All strains were cultivated in minimal medium with the specific substrates. Data are presented as mean ± s.e.m. (n = 3 biologically independent samples)