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Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: Towards green biomanufacturing of high-value recombinant proteins using promising cell factory: Chlamydomonas reinhardtii chloroplast

Fig. 2

Schematic diagram of the marker-free transgenic strategy. LA left homologous arm; GOI gene of interest; DM dual marker; RA right homologous arm; PS photosynthesis; PTXD phosphite dehydrogenase; Phi phosphite; R resistance marker; A restoration of photoautotrophic defects. B Introduction of function-added selectable marker. C Propel antibiotic marker gene loss by negative pressure selection. D Homologous recombination of foreign genes into the genome to replace antibiotic marker gene

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