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Bioresources and Bioprocessing

Table 1 Kinetic constants of AcCO and its variants in producting CHDA

From: Efficient synthesis 1,4-cyclohexanedicarboxaldehyde by an engineered alcohol oxidase

Strains

aSpecific activityCHDA (U·g−1)

KMCHDA (mM)

kcatCHDA (min−1)

bkcat/KMCHDA (mM−1·min−1)

WT

0.29 ± 0.097

26.81 ± 0.19

0.043 ± 0.013

1.60 × 10–3

W1(S101A)

0.95 ± 0.25

20.42 ± 0.12

0.14 ± 0.022

6.86 × 10–3

W1*(H351V)

1.24 ± 0.14

16.24 ± 0.11

0.24 ± 0.022

1.48 × 10–2

W2(S101A/H351V)

2.43 ± 0.13

14.44 ± 0.46

0.41 ± 0.014

2.84 × 10–2

W3(S101A/H351V/N378S)

2.87 ± 0.43

6.81 ± 0.13

0.53 ± 0.12

7.78 × 10–2

W4(S101A/H351V/N378S/Q329N)

4.21 ± 0.38

5.24 ± 0.28

0.94 ± 0.043

0.18

  1. aThe specific activity of CHDA was determined with 10 μM purified AcCO (WT) or its variants and 5 mM HMCA in 1 mL air-saturated potassium phosphate buffer (100 mM, pH 8.0) at 30 °C for 30 min
  2. bThe kcat/KM values of CHDA was determined with 10 μM purified AcCO (WT) or its variants and 1–100 mM HMCA in 1 mL air-saturated potassium phosphate buffer (100 mM, pH 8.0) at 30 °C for 30 min
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