Fig. 3

Time course of the 100-mL preparative-scale multi-enzyme cascade reaction. Step I: 10 mM (−)-limonene (with 2% DMSO), 15 g_cdw L⁻¹ A7F resting cells, 0.2 mM NAD⁺, 100 mM sodium formate, 1 mg/mL Triton X-100, KPB buffer (100 mM, pH 7.5), 25 °C, 200 rpm. Step II: supplement of 0.2 U mL⁻¹ LkADH, 1 U mL⁻¹ SmNOX, 2 U mL⁻¹ ATA-117, 80 mM 2-pentanamine, 0.2 mM NAD⁺ and 0.2 mM PLP, incubated at 35 °C and shaken at 200 rpm. Concn.: concentration