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Bioresources and Bioprocessing
Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: Bioeconomic production of high-quality chitobiose from chitin food wastes using an in-house chitinase from Vibrio campbellii

Fig. 1

Expression, purification and chitinase activity determination of VhChiA. a SDS-PAGE analysis. VhChiA was eluted from a Ni–NTA agarose affinity column with 150 mM imidazole in the equilibration buffer and its purity was assessed by 12% SDS-PAGE. kDa, protein markers; Lane 1, crude extract; 2, unbound fraction; 3, fraction washed with the equilibration buffer; 4–7, fractions eluted with 150 mM imidazole; 8, last fraction washed with the equilibration buffer. b Michaelis–Menten plot of the initial rate (v0) vs. pNP-(GlcNAc)2 concentration [S]

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