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Bioresources and Bioprocessing
Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: Bioeconomic production of high-quality chitobiose from chitin food wastes using an in-house chitinase from Vibrio campbellii

Fig. 2

Time-course of hydrolysis of various types of chitins by VhChiA. a β-chitin (squid pen), b α-chitin (shrimp shell), c α-chitin (crab shell) and d crude chitooligosaccharide mixture. Small aliquots of the reaction sample from time points 0, 2.5, 5, 10, 30 min and 1,16, 24 h were analyzed by TLC. Each reaction mix contained 5 mg (dry weight) of different colloidal chitins in 0.1 M sodium acetate buffer pH 5.5 and 100 U of VhChiA. The control was substrate alone. Std represents a mixture of (GlcNAc)1–6 (labeled DP1–DP6), migrating alongside the reaction samples. Control lanes (C) contained the substrate but no enzyme

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