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Table 1 Central composite design and experimental data used for the response surface analysis

From: Vitamin combination promotes ex vivo expansion of NK-92 cells by reprogramming glucose metabolism

Runs

Factors

Total cell expansion, Y

Nicotinamide(µM) A

Riboflavin(µM) B

Inositol(µM)

C

1

−1 (21.26)

−1 (0.48)

1 (195.82)

57.51

2

−1 (21.26)

−1 (0.48)

−1 (69.90)

79.80

3

1 (35.24)

1 (1.10)

−1 (69.90)

43.74

4

−1 (21.26)

1 (1.10)

1 (195.82)

63.04

5

1 (35.24)

1 (1.10)

1 (195.82)

56.83

6

0 (28.25)

0 (0.79)

0 (132.86)

94.57

7

0 (28.25)

0 (0.79)

0 (132.86)

83.48

8

−1 (21.26)

1 (1.10)

−1 (69.90)

44.48

9

0 (28.25)

0 (0.79)

0 (132.86)

99.52

10

1 (35.24)

−1 (0.48)

−1 (69.90)

76.27

11

0 (28.25)

0 (0.79)

0 (132.86)

89.47

12

1 (35.24)

−1 (0.48)

1 (195.82)

80.05

13

0 (28.25)

0 (0.79)

0 (132.86)

86.55

14

0 (28.25)

−1.69 (0.26)

0 (132.86)

64.49

15

1.69 (40.00)

0 (0.79)

0 (132.86)

72.04

16

−1.69 (16.50)

0 (0.79)

0 (132.86)

60.16

17

0 (28.25)

0 (0.79)

0 (132.86)

82.08

18

0 (28.25)

0 (0.79)

−1.69 (26.97)

72.39

19

0 (28.25)

1.69 (1.31)

0 (132.86)

55.05

20

0 (28.25)

0 (0.79)

1.69 (238.75)

54.22