Fig. 6From: Two strategies to improve the supply of PKS extender units for ansamitocin P-3 biosynthesis by CRISPR–Cas9Transcription analysis of the secondary metabolic synthesis gene cluster in strain L40. Transcription analysis of the putative gene PKS clusters in strain L40. The 2−ΔΔCt method was used to calculate the expression level, the average expression value of asmC is set to 1 as the standard. Fermentation experiments were performed three times. The values are means ± SD (standard deviation) of three independent experiments. Significant differences were analyzed by one-way ANOVA, and **p < 0.01, ****p < 0.0001Back to article page