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Bioresources and Bioprocessing
Fig. 9 | Bioresources and Bioprocessing

Fig. 9

From: Two strategies to improve the supply of PKS extender units for ansamitocin P-3 biosynthesis by CRISPR–Cas9

Fig. 9

Transcript assay of AP-3 biosynthetic gene cluster (A) and transcription unit distribution (B). A RNA sample was isolated from day 3 culture of strain L40. RT-PCR fragments were shown. (asm11-12, 374 bp; asm14-15, 341 bp; asm15-16, 513 bp; asm16-17, 435 bp; asm8-C, 362 bp; asm2-3-4, 854 bp; asm4-5, 566 bp; asmC-D, 589 bp; asmD-9, 348 bp; asmA-B, 482 bp; asm18-19, 402 bp; asm34-35, 617 bp). B Horizontal black arrows show the transcription unit confirmed by RT-PCR. ‘x’, no mRNA connecting the genes. asm11 shares 4 bp with asm10, asm13 shares 4 bp with asm14

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