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Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: Development of highly efficient whole-cell catalysts of cis-epoxysuccinic acid hydrolase by surface display

Fig. 1

CESH[L] surface display efficiencies of different anchoring motifs. a SDS-PAGE analysis of the whole cells or the cell lysate with and without trypsin treatment. Lanes 1–2, whole cells of intracellular CESH[L] expression system without and with trypsin treatment; Lane 3, trypsin-treated cell lysate of the intracellular CESH[L] expression system. Lanes 4–13, each pair of lanes are trypsin-untreated and treated whole cells expressing LOA-CESH[L], MipAV140-CESH[L], YiaTR232-CESH[L], InaKN-CESH[L], and InaPbN-CESH[L], respectively. Lanes M, standard protein molecular weight markers. b Whole-cell activities of various CESH[L] expression systems incubated at 4 °C for different days. c Comparison of the surface display systems and intracellular expression system. Intra, LOA, MipA, YiaT, InaKN, and InaPbN represent the cells expressing intracellular CESH[L], LOA-CESH[L], MipAV140-CESH[L], YiaTR232-CESH[L], InaKN-CESH[L], and InaPbN-CESH[L], respectively. Intra-L, Intra-T, and Intra-T-S represent the whole-cell lysate, the Triton X-100 treated cells, and the supernatant after the Triton X-100 treatment, respectively, of the intracellular expression system. The activities of all surface display systems were measured after 2-day incubation at 4 °C, while the activities of the intracellular expression system were measured without incubation. Symbols ** indicate significant differences with p-values less than 0.01

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