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Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: Development of highly efficient whole-cell catalysts of cis-epoxysuccinic acid hydrolase by surface display

Fig. 2

SDS-PAGE analysis of the InaPbN-CESH[L] expression. a The effect of the inducer IPTG concentrations. Lanes 1–12, each pair of lanes are trypsin-untreated and treated whole cells induced by IPTG with the concentrations of 0.0 mM, 0.2 mM, 0.4 mM, 0.6 mM, 0.8 mM, and 1.0 mM. Lane M, standard protein molecular weight markers. b The effect of the expression temperature. Lanes 1–10, each pair of lanes are trypsin-untreated and treated whole cells with the expression temperatures 16 °C, 20 °C, 25 °C, 30 °C, and 37 °C. Lane M, standard protein molecular weight markers. c The whole-cell enzymatic activities of InaPbN-CESH[L] with different expression temperatures. d Fraction analysis of intracellular expressed CESH[L] (lanes 1–3) and displayed InaPbN-CESH[L] (lanes 4–6). W, whole-cell lysate; S, supernatant after centrifugation of the cell lysate; P, pellet after centrifugation of the cell lysate. Lane M, standard protein molecular weight markers

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