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Table 1 Plasmids used in this study

From: Development of highly efficient whole-cell catalysts of cis-epoxysuccinic acid hydrolase by surface display

Plasmids

Descriptions

Source

pET28a

pBR232 origin, lacI coding sequence, Kmr, PT7

Novagen

pET22b

pBR232 origin, lacI coding sequence, Apr, PT7

Novagen

pET28a-CESH[L]

pET28a derivative, Kmr, intracellular CESH[L] expression with N-terminal His6-tag

(Cui et al. 2012)

pET28a-INPN-SC3

pET28a derivative, Kmr, source of InaKN gene

Gift from Prof. Chengzhi He

pET28a-InaPbN

pET28a derivative, Kmr, source of InaPbN gene

(Song et al. 2015)

pET22b-LOA-CESH[L]

pET22b derivative, PT7, Apr, fusion expression of LOA-CESH[L]

This study

pET22b-MipAV140-CESH[L]

pET22b derivative, PT7, Apr, fusion expression of MipAV140-CESH[L]

This study

pET22b-YiaTR232-CESH[L]

pET22b derivative, PT7, Apr, fusion expression of YiaTR232-CESH[L]

This study

pET22b-InaKN-CESH[L]

pET22b derivative, PT7, Apr, fusion expression of InaKN-CESH[L]

This study

pET28a-InaPbN-CESH[L]

pET28a derivative, PT7, Kmr, fusion expression of InaPbN-CESH[L]

This study