An accessory enzymatic system of cellulase for simultaneous saccharification and co-fermentation

Liu, Han; Wang, Xuxin; Liu, Yanping; Kang, Zhuoran; Lu, Jiaqi; Ye, Yutong; Wang, Zhipeng; Zhuang, Xinshu; Tian, Shen

doi:10.1186/s40643-022-00585-5

Table 4 Summaries of SSCF performance of engineered yeast consortium

From: An accessory enzymatic system of cellulase for simultaneous saccharification and co-fermentation

Engineered yeast consortium	Maximum ethanol concentration** (g/l)	Theoretical ethanol yield^a (%)	Substrate consumption rate (g/l/h)^d	Cellulose conversion rate^b (%)	Hemicellulose conversion rate^c (%)
WA1^g/ScafI + WA1^g/XA + WA1^g/Xn	12.88 ± 0.64	84.87 ± 0.26	0.31 ± 0.01	91.21 ± 0.89	55.25 ± 0.37
WA1^g/NC + WA1^g/XA + WA1^g/Xn	12.48 ± 0.74	86.04 ± 0.34	0.30 ± 0.02	90.14 ± 0.71	49.37 ± 0.44
WA1^g/NC	11.47 ± 0.70	86.92 ± 0.32	0.27 ± 0.01	86.41 ± 0.59	-

^a\({\text{Ethanol}}\,{\text{yield}}\,\left( \% \right) = \frac{{{\text{produced}}\,{\text{ethanol}}\,\left( {\text{g}} \right)}}{{{\text{consumed}}\,{\text{glucose}}\,\left( {\text{g}} \right) \times 0.51 + {\text{consumed}}\,{\text{xylose}}\left( {\text{g}} \right) \times 0.46}} \times 100\%\)
^b\({\text{Cellulose}}\,{\text{conversation}}\,{\text{yield}}\left( \% \right) = \frac{{{\text{Cellulose}}_{{\text{s}}} \left( {\text{g}} \right) - {\text{Cellulose}}_{{\text{R}}} \left( {\text{g}} \right)}}{{{\text{Cellulose}}_{{\text{s}}} \left( {\text{g}} \right)}} \times 100\% \,\left( {{\text{Cellulose}}_{{\text{s}}} {\text{:cellulose}}\,{\text{from}}\,{\text{total}}\,{\text{substrate;}}\,{\text{Cellulose}}_{{\text{R}}} {:}\,{\text{cellulose}}\,{\text{from}}\,{\text{fermentation}}\,{\text{residues}}} \right)\)
^c\({\text{Hemicellulose}}\,{\text{conversation}}\,{\text{yield}}\left( \% \right) = \frac{{{\text{Hemicellulose}}_{{\text{s}}} \left( {\text{g}} \right) - {\text{Hemicellulose}}_{{\text{R}}} \left( {\text{g}} \right)}}{{{\text{Hemicellulose}}_{{\text{s}}} \left( {\text{g}} \right)}} \times 100\% \,\left( {{\text{Hemicellulose}}_{{\text{s}}} :{\text{hemicellulose}}\,{\text{from}}\,{\text{total}}\,{\text{substrate}};\,{\text{Hemicellulose}}_{{\text{R}}} :{\text{hemicellulose}}\,{\text{from}}\,{\text{fermentation}}\,{\text{residues}}} \right)\)
^d\({\text{Substrate}}\,{\text{consumption}}\,{\text{rate}}\,\left( {\text{g/l/h}} \right) = \frac{{{\text{Consumed}}\,{\text{cellulose}} \times 1.1\,\left( {\text{g/l}} \right) - {\text{Consumed}}\,{\text{hemicellulose}} \times 1.136\left( {\text{g/l}} \right)}}{{96\left( {\text{h}} \right)}}\)
^**Indicates statistically significant differences between experimental group and control group (T test, p < 0.01)

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