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Table 1 Effect of explant type and 2,4-D concentration on callus induction (%) after 4 weeks of growth

From: Effect of explant type (leaf, stem) and 2,4-D concentration on callus induction: influence of elicitor type (biotic, abiotic), elicitor concentration and elicitation time on biomass growth rate and costunolide biosynthesis in gazania (Gazania rigens) cell suspension cultures

Origin of callus 2,4-D (mg/L) Callus induction (%)
Stem 0.0 50.0 ± 5.8b(C)
Stem 0.5 70.0 ± 0.0ab(B)
Stem 1.0 80.0 ± 10.0a(AB)
Stem 1.5 90.0 ± 5.8a(A)
p values (one-way ANOVA) (stem explants, 2,4-D concentration) 0.013*
Leaf 0.0 60.0 ± 5.8a(BC)
Leaf 0.5 70.0 ± 5.8a(B)
Leaf 1.0 80.0 ± 5.8a(AB)
Leaf 1.5 70.0 ± 10.0a(B)
p values (one-way ANOVA) (leaf explants, 2,4-D concentration) 0.330 ns
p values (two-way ANOVA/general linear model)
 2,4-D concentration (A) 0.006**
 Explant type (B) 0.045*
 (A)*(B) 0.020*
  1. Means (n = 30) ± standard error (S.E.) with the same letter in a column are not statistically significant different from each other according to the Duncan’s multiple range test at p ≤ 0.05. ns p > 0.05; *p ≤ 0.05; **p ≤ 0.01. Superscript small letters—differences between four 2,4-D concentrations for each explant type (either stem or leaf) separately (one-way ANOVA). Superscript capital letters in parenthesis—differences between samples from the combined effect of two explant types (leaf, stem) and four 2,4-D concentrations (two-way ANOVA)